What does chloroquine do in transfection

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  1. Pavel G Moderator

    What does chloroquine do in transfection

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    Transfection is the process of introducing nucleic acids into eukaryotic cells by nonviral methods. Using various chemical or physical methods, this gene transfer technology enables the study of gene function and protein expression in a cellular environment. Nov 25, 2019 Follow all directions on your prescription label. Do not take chloroquine in larger or smaller amounts or for longer than recommended. To prevent malaria Start taking the medicine 2 weeks before entering an area where malaria is common. Chloroquine is usually taken once per week on the same day each week. So far we detected much higher transfection efficiency with Chloroquine than conventional CaPO4 transfection at 24 h. We use two different conditions with %2 FBS and %10 FBS. At the first day %10.

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    What does chloroquine do in transfection

    Structure−Function Correlation of Chloroquine and Analogues., Chloroquine Uses, Side Effects & Warnings -

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  7. It was originally used as an antimalarial compound. It kills the erythrocytic forms of malaria parasites at all stages of development, but does not affect the sporozo ites. Keywords chloroquine diphosphate salt, c6628, c18h26cin3, dna transfection, tranfection efficiency, antigen-antibody complexes dissociate, denaturing red blood cell antigens,

    • Chloroquine diphosphate salt - Sigma-Aldrich.
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    • Helper Dependent Protocol - Stanford University.

    Transfection efficiencies will vary with cell type Can be used only for transient transfection Typically produces less than 10% delivery in primary cells Chloroquine works best when you take it on a regular schedule. For example, if you are taking it once a week to prevent malaria, it is best to take it on the same day of each week. Make sure that you do not miss any doses. If you have any questions about this, check with your doctor. Chloroquine is an inhibitor of the lysosomal degradation of the DNA which is taken up by the cells, so as leelee said, transfection should have been successful, albeit at a slightly lower level than if you would have added the chloroquine. In my normal transfections, I never add chloroquine and get high efficiencies nevertheless.

  8. ludmila240 Well-Known Member

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  10. romarkill XenForo Moderator

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