Chloroquine dna supercoiling

Discussion in 'Hydroxychloroquine Sulfate 200 Mg' started by SMRT, 08-Mar-2020.

  1. mView User

    Chloroquine dna supercoiling


    Supercoiling is important in a number of biological processes, such as compacting DNA, and by regulating access to the genetic code, DNA supercoiling strongly affects DNA metabolism and possibly gene expression. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription.

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    The type IIA subfamily of topoisomerases both resolves DNA entanglements and regulates DNA supercoiling through a strand passage mechanism whereby one DNA duplex, the transfer T segment, is transported through a double-stranded break in a second DNA, the gate G segment 4,7. Ofthe samples shownare migrating as positively supercoiled DNA, as determined by comparison with a second gel containing less chloroquine. FIG. 2. Supercoiling ofpBR322as a function ofbound histone octamers. O/Pis the number of octamers per plasmid. For each histone-to-DNA ratio, supercoiling was carried out at three gyrase Positively supercoiled DNA is generated when chloroquine binds to very relaxed molecules, and these topoisomers can be resolved using two‐dimensional chloroquine gels; here, a higher chloroquine concentration 25 µg ml −1 in the second dimension causes the positively supercoiled DNA to migrate faster than negatively supercoiled DNA.

    Adding or subtracting twists, as some enzymes can do, imposes strain. In a "relaxed" double-helical segment of B-DNA, the two strands twist around the helical axis once every 10.4–10.5 base pairs of sequence.

    Chloroquine dna supercoiling

    DNA Topology Fundamentals - Tufts University, Supercoiling on the of

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  5. In our study, we used an intercalating drug, chloroquine, which is known to decrease the twist of the double helix and thus altered the conformation of the whole DNA. During our experiments, a chloroquine solution was added while imaging a few highly condensed plasmid nanoparticles in solution.

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    The supercoiling state of plasmid pUC18 was analysed primarily by means of one‐dimensional agarose gels containing 2.5 µg ml −1 chloroquine, which intercalates with DNA and relaxes negative supercoils. Supercoiled molecules are more compact and so migrate faster through the gel. DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA, and by regulating access to the genetic code, DNA supercoiling strongly affects DNA metabolism and possibly gene expression. Galactose for 2 hr. DNA was isolated and the topology of the plasmid analyzed using gel electrophoresis in the presence of chloroquine. This technique resolves nega- ’ tively supercoiled plasmid DNA into a distribution of topoi- Somers which reflects the heterogeneity of plasmid DNA

     
  6. Flashstile Moderator

    This article focuses upon hydroxychloroquine retinopathy. Chloroquine was originally used as an anti-malarial therapeutic. Chloroquine Retinopathy Is Fluorescein Angiography Necessary. Macular Telangiectasia A cause of bull’s eye maculopathy Chloroquine-induced bull’s eye maculopathy
     
  7. Gabriel Well-Known Member

    This article focuses upon hydroxychloroquine retinopathy. Chloroquine was originally used as an anti-malarial therapeutic. Hydroxychloroquine-Induced Retinal Toxicity - American. Wrong Hydroxychloroquine Dose Is Common, Putting Eyes at Risk. Hydroxychloroquine Plaquenil Toxicity and Recommendations.
     
  8. juginka New Member

    Hydroxychloroquine Plaquenil Success, Reviews and Side Effects Long-term hydroxychloroquine use has been associated with retinal toxicity, although patients with rheumatologic conditions often discontinue hydroxychloroquine therapy due to inadequate disease control rather than toxicity.

    Continuation of long term treatment with hydroxychloroquine in systemic.